HT-29-EGFP cell line

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HT-29-EGFP Order

HT-29-EGFP cell line
Catalog#
YC-C008-EGFP-P
Size
1*10^6
Transfection Culture Medium
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
HT-29-EGFP cell line
Order

Detailed Product Information

Product category
EGFP Stable Cell Lines
cell name
HT-29-EGFP
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Reporter gene
EGFP
Resistance gene
puro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%McCOY's 5A+10% FBS
Loci STR Info (Sample Cell)
Sample Cell Line:HT-29
STR Info (Cell bank)
Cell Line:HT-29
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 11121112
D7S820 10101010
D16S539 11121112
VWA 17191719
TH01 6969
AMEL XXXX
TPOX 8989
CSF1PO 11121112
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.
EGFP validation
HT-29-EGFP cell line EGFP validation

Instruction

Use EGFP gene expressing lentivirus constructed by Ubigene to infect the HT-29 cell line, screen the cells based on the puromycin resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a HT-29-EGFP cell line that can maintain long-term, stable and high expression.

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